Adeegga Kahortagga Jirka ee Monoclonal
Monoclonal antibody, oo ay soo saartay hal unug B-clone ah, ayaa leh mid aad u sarreeya kaas oo bartilmaameedsada epitope antigen gaar ah.Monoclonal antibody wuxuu leeyahay faa'iidooyin badan, oo ay ku jiraan daahirnimo sare, dareen, iyo gaar ahaan.Hybridomas waxaa lagu abuuraa isku-dhafka B-lymphocytes kuwaas oo soo saara unugyo gaar ah oo gaar ah, oo leh unugyo myeloma ah oo cimri dheer.Bioantibody waxay isticmaashaa tignoolajiyada fiyuuska ee waxtarka sare leh oo 20 jeer ka waxtar badan hababka fiyuuska caadiga ah.Intaa waxaa dheer, waxay shaqaaleysiisaa tignoolajiyada baarista microarray borotiinka si ay u aqoonsato unugyada unugyada monoclonal ee muujiya gaar gaar ah, isku xirnaanta, iyo waxtarka shaqada ee ka dhanka ah epitopes gaarka ah.
| Alaabta adeegga | Waxyaabaha Tijaabada ah | Waqtiga Hogaaminta (Todobaadka) |
| Diyaarinta antigen | 1. Macmiilku wuxuu bixiyaa antigen-ka2. Bioantibody waxay diyaarisaa antigen-ka | / |
| Talaalka jiirka | Tallaalka jiirka BALB/c, ururinta serum iyo falanqaynta ELISA | 4 |
| Isku-dhafka unugga iyo baaritaanka | Fusion of mouse splenocytes iyo unugyada myeloma, baaritaanka koofiyadda | 2 |
| Samaynta khadka gacanta ee xasiloon | Subcloning of clones togan ee la baadhay | 3 |
| Aqoonsiga isotype antibody | Aqoonsiga noocyada hoose ee xariiqda unugyada | 1 |
| Kalluumaysiga miisaan yar | Ku-soo-bax aan serum lahayn | 2 |
| Ku-soo-bax ballaaran iyo nadiifin | 200 ml oo ah ka-bax la'aan iyo nadiifin | 1 |
