Muujinta borotiinka Unugga Khamiirka
Nidaamka muujinta khamiirka waa hab si ballaaran loo isticmaalo muujinta borotiinka eukaryotic, taas oo ay ugu wacan tahay fududaantiisa beerashada, la awoodi karo, iyo sahlanaanta hawlgalka.Ka mid ah noocyada khamiirka ee kala duwan, Pichia pastoris waa martigeliyaha ugu caansan ee martigeliyaha, maadaama ay fududeyso muujinta borotiinka gudaha iyo unugyada ka baxsan labadaba.Nidaamku waxa kale oo uu suurtageliyaa wax ka beddelka tarjumaada ka dib, sida fosforyaalka iyo glycosylation, taasoo keentay hab muujinta eukaryotic gaar ah oo leh faa'iidooyin badan.
| Alaabta adeegga | Waqtiga Hogaaminta (BD) |
| Kordhinta Codon, isku-dhafka hidde-sideyaasha iyo subcloning | 5-10 |
| Baaritaanka clone togan | 10-15 |
| Muujinta cabbirka yar | |
| Miisaanka weyn (200ML) muujinta iyo nadiifinta, wax-soo-saarka waxaa ka mid ah borotiinka la safeeyey iyo warbixinta tijaabada ah |
Haddii hidda-wadaha lagu farsameeyo Bioantibody, plasmid-ka la dhisay ayaa lagu dari doonaa waxyaalaha la keeni karo.