Muujinta borotiinka Unugyada Cayayaanka
Nidaamka muujinta unugga cayayaanka waa hab caadi ah oo loo isticmaalo habka odhaahda eukaryotic si loogu muujiyo borotiinno-molecular-weyn.Marka la barbardhigo unugyada naasleyda, xaaladaha dhaqanka unugga cayayaanka ayaa ah kuwo fudud oo aan u baahnayn CO2.Baculovirus waa nooc ka mid ah fayras DNA-da laba-geesoon leh oo leh unugyo cayayaan sida martigeliyaha dabiiciga ah.Waxay leedahay gaar ahaan noocyada sare, ma qaadsiiso laf dhabarta, dhibna uma laha dadka iyo xoolaha.sf9, oo ah kan ugu badan ee loo isticmaalo sida unugga martida loo yahay, ayaa ka muuqda planktonic ama ku dheggan dhaqanka.sf9 aad bay ugu habboon tahay muujinta baaxadda weyn, waxaana loo isticmaali karaa habaynta dambe iyo beddelka borotiinnada sida phosphorylation, glycosylation, iyo acylation.Nidaamka muujinta unugyada cayayaanka ayaa sidoo kale loo isticmaali karaa muujinta hiddo-wadaha badan, sidoo kale wuxuu muujin karaa borotiinnada sunta ah sida peptides antimicrobial.
| Alaabta adeegga | Waqtiga Hogaaminta (BD) |
| Kordhinta Codon, isku-dhafka hidde-sideyaasha iyo subcloning | 5-10 |
| Fayraska jiilka P1 iyo muujinta cabbirka yar | 10-15 |
| Fayraska jiilka P2, muujinta cabirka weyn iyo nadiifinta, gaarsiinta borotiinka la safeeyey iyo warbixinta tijaabada ah |